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V N Anisimov A V Arutiunian T I Oparina S O Burmistrov V M Prokopenko V Kh Khavinson 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》1999,85(4):502-507
Activity of Cu,Zn-superoxide dismutase (SOD) in old rats' brain was found to be decreased by 46.8% as compared to young animals. The brain concentration of Schiff bases (SB) was decreased by 13.6% in old rats, whereas concentration of diene conjugates (DC), protein peroxidation (PP), and total antioxidative activity (TAA) was the same in old as well as in young rats. The liver level of the DC and TAA was also the same. The serum level of the PP, SB, and DC was increased whereas the activity of the SOD and TAA was decreased in old rats. The findings suggest occurrence of considerable age-related changes in free radical processes as well as the organ specifics of these changes in rats. 相似文献
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Mutations in the highly conserved GGQ motif of class 1 polypeptide release factors abolish ability of human eRF1 to trigger peptidyl-tRNA hydrolysis. 总被引:10,自引:1,他引:9 下载免费PDF全文
L Y Frolova R Y Tsivkovskii G F Sivolobova N Y Oparina O I Serpinsky V M Blinov S I Tatkov L L Kisselev 《RNA (New York, N.Y.)》1999,5(8):1014-1020
Although the primary structures of class 1 polypeptide release factors (RF1 and RF2 in prokaryotes, eRF1 in eukaryotes) are known, the molecular basis by which they function in translational termination remains obscure. Because all class 1 RFs promote a stop-codon-dependent and ribosome-dependent hydrolysis of peptidyl-tRNAs, one may anticipate that this common function relies on a common structural motif(s). We have compared amino acid sequences of the available class 1 RFs and found a novel, common, unique, and strictly conserved GGQ motif that should be in a loop (coil) conformation as deduced by programs predicting protein secondary structure. Site-directed mutagenesis of the human eRF1 as a representative of class 1 RFs shows that substitution of both glycyl residues in this motif, G183 and G184, causes complete inactivation of the protein as a release factor toward all three stop codons, whereas two adjacent amino acid residues, G181 and R182, are functionally nonessential. Inactive human eRF1 mutants compete in release assays with wild-type eRF1 and strongly inhibit their release activity. Mutations of the glycyl residues in this motif do not affect another function, the ability of eRF1 together with the ribosome to induce GTPase activity of human eRF3, a class 2 RF. We assume that the novel highly conserved GGQ motif is implicated directly or indirectly in the activity of class 1 RFs in translation termination. 相似文献
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I. A. Anikhovskaya O. N. Oparina M. M. Yakovleva M. Yu. Yakovlev 《Human physiology》2006,32(2):200-203
Intestinal endotoxin is an obligate adaptive and pathogenetic factor of general adaptation syndrome. In athletes, systemic blood endotoxin levels, regulated by the sympathoadrenal system, increase as the athletic performance is enhanced and the exercise tolerance depends on the ability of the body to consume this endotoxin and on the extent to which it is absorbed in the intestine. 相似文献
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O. A. Ten O. R. Borodulina N. S. Vassetzky N. Iu. Oparina D. A. Kramerov 《Molecular Biology》2006,40(1):63-71
Copies of two repetitive elements of the common tree shrew (Tupaia glis) genome were cloned and sequenced. The first element, Tu III, is a ~260 bp long short interspersed element (SINE) with the 5′ end derived from glycine RNA. Tu III carries long polypurine-and polypyrimidine-rich tracts, which may contribute to the specific secondary structure of Tu III RNA. This SINE was also found in the genome of the smooth-tailed tree shrew of another genus (Dendrogale). Tu III appears to be confined to the order Scandentia since it was not found in the DNA of other tested mammals. The second element, Tu-SAT1, is a tandem repeat with a monomer length of 365 bp. Some properties of its nucleotide sequence suggest that Tu-SAT1 is a centromeric satellite. 相似文献
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Invariant amino acids essential for decoding function of polypeptide release factor eRF1 总被引:2,自引:2,他引:0
Kolosov P Frolova L Seit-Nebi A Dubovaya V Kononenko A Oparina N Justesen J Efimov A Kisselev L 《Nucleic acids research》2005,33(19):6418-6425
In eukaryotic ribosome, the N domain of polypeptide release factor eRF1 is involved in decoding stop signals in mRNAs. However, structure of the decoding site remains obscure. Here, we specifically altered the stop codon recognition pattern of human eRF1 by point mutagenesis of the invariant Glu55 and Tyr125 residues in the N domain. The 3D structure of generated eRF1 mutants was not destabilized as demonstrated by calorimetric measurements and calculated free energy perturbations. In mutants, the UAG response was most profoundly and selectively affected. Surprisingly, Glu55Arg mutant completely retained its release activity. Substitution of the aromatic ring in position 125 reduced response toward all stop codons. This result demonstrates the critical importance of Tyr125 for maintenance of the intact structure of the eRF1 decoding site. The results also suggest that Tyr125 is implicated in recognition of the 3d stop codon position and probably forms an H-bond with Glu55. The data point to a pivotal role played by the YxCxxxF motif (positions 125–131) in purine discrimination of the stop codons. We speculate that eRF1 decoding site is formed by a 3D network of amino acids side chains. 相似文献